Fig. 4: ALDH16A1 interacts with ferroptosis suppressor TXN. | Nature Communications

Fig. 4: ALDH16A1 interacts with ferroptosis suppressor TXN.

From: Targeting ALDH16A1 mediated thioredoxin lysosomal degradation to enhance ferroptosis susceptibility in SMARCA4-deficient NSCLC

Fig. 4: ALDH16A1 interacts with ferroptosis suppressor TXN.The alternative text for this image may have been generated using AI.

a Flag and TXN proteins immunoprecipitated with Flag-ALDH16A1 and total lysates used for immunoprecipitation (IP). b Representative images of PLA signals between endogenous ALDH16A1 and TXN in PC9 and A549 cells, as detected by Duolink PLA assay. Scale bars: 5 μm. c Schematic depiction of TXN-based antioxidative system. d Cell viability in PC9 and A549 cells treated with RSL3 for 6 h or IKE (PC9: 2 μM; A549: 40 μM) for 24 h following pre-treatment with 1 μM auranofin, PX-12, or conoidin A for 6 h. e Cell viability in PC9 and A549 cells treated with 10 μM auranofin, PX-12, or conoidin A combined with or without DFO (100 μM) or Fer-1 (10 μM) for 24 h. f Lipid peroxidation in PC9 and A549 cells treated with RSL3 for 3 h following pre-treatment with 1 μM auranofin for 6 h. g Cell viability in PC9, H1975, A549 and H23 cells with indicated genotypes treated with RSL3 for 6 h following pre-treatment with auranofin for 6 h. h Relative TXN activity in PC9 and A549 cells with indicated genotypes determined by fluorometric assay. i ALDH16A1 and TXN protein levels in PC9, H1975, A549 and H23 cells with indicated genotypes. j SMARCA4, ALDH16A1 and TXN protein levels in PC9, H1975, A549 and H23 cells with indicated genotypes. k SMARCA4, ALDH16A1 and TXN protein levels in SMARCA4 (wt) and deficient NSCLC tumour tissues determined by immunohistochemistry (Scale bars: 100 μm, n = 37). l mRNA levels of TXN in PC9, H1975, A549 and H23 cells with indicated genotypes. m TXN protein levels in PC9, H1975, A549 and H23 cells with indicated genotypes treated with 100 μg/mL CHX for 0, 4 or 8 h. n TXN protein levels in PC9, H1975, A549 and H23 cells with indicated genotypes treated with 100 μg/mL CHX combined with or without 10 μM CQ or MG132 for 8 h. CQ, chloroquine. Data are presented as the mean ± SD, n = 3 independent experiments. Consistent results were observed across three biological replicates in (a, b, i, j, k, m, n). Unpaired two-tailed Student’s t tests are used. Source data are provided as a Source Data file.

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