Fig. 6: Characterization of adrenocortical lymphocytes. | Nature Communications

Fig. 6: Characterization of adrenocortical lymphocytes.

From: Impact of steroid differentiation on tumor microenvironment revealed by single-nucleus atlas of adrenal tumors

Fig. 6

Individual lymphoid cells from 34 adrenocortical tumors and 4 normal adrenals are presented (single-nucleus transcriptomes). a UMAP annotated by transcriptome clusters. b Stacked violin plot of transcriptome cluster markers, showing log-scaled raw counts for top differentially expressed genes for each cluster. c Proportion of transcriptome clusters in each sample type. d Lymphocytes trajectory annotated by pseudotime, using discriminative dimensionality reduction via learning a tree (DDRtree). e Lymphocytes trajectory annotated by transcriptome clusters, using DDRtree. f Expression profiles of top genes with pseudotime variation, from unassigned T cells towards NK-like T cells (solid line), including NK differentiation markers (KLRD1, KLRF1 and GNLY). Black lines: mean expression over pseudotime. g Expression profiles of top genes with pseudotime variation, from unassigned T cells towards exhausted T cells (dashed line), including THEMIS, a negative regulator of TCR signaling. Black lines: mean expression over pseudotime.

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