Fig. 3: CRACI assigns ‘writer’ protein to each D-modified site in human tRNA.
From: Quantitative CRACI reveals transcriptome-wide distribution of RNA dihydrouridine at base resolution
a An integrated overview of DUS-dependent D profiles in HepG2 ct-tRNAs. Comparative analysis of D stoichiometry dynamics at specific sites within HepG2 ct-tRNAs following 72-hour siRNA-mediated knockdown of 4 DUS enzymes: b D16/17 (n = 26), c D20 (n = 27), d D47 (n = 25), and e D20a (n = 16). P-values from two-sided paired t-tests are displayed. Each point was calculated as the average of at least two biological replicates. f Integrated overview of DUS2L-regulated D sites in HepG2 mt-tRNAs. g Comparison of D modification levels in mt-tRNAs in DUS2L knockdown versus the control. Two biological replicates are used for control samples and three biological replicates are used for DUS2L knockdown samples. P-values from two-sided unpaired t-tests are displayed. h Comparison of tRNA expression levels in response to depletion of 4 DUS enzymes, with tRNAs categorized into DUS-targets versus non-DUS targets. Expression levels of different RNA species were calculated as the average of at least two biological replicates. P-values from two-sided unpaired t-tests are displayed.
