Fig. 5: CRACI uncovers D landscape in Mus musculus (mESC) and Arabidopsis thaliana.
From: Quantitative CRACI reveals transcriptome-wide distribution of RNA dihydrouridine at base resolution
a The identified D sites in ct-tRNAs from mouse embryonic stem cells (mESC) using CRACI. b Comparison of modification levels at specific D sites in mESC ct-tRNAs, with violin plots representing the distribution of D stoichiometry at different positions of tRNAs. The D fraction of each site was calculated as the average of two biological replicates. c The identified D sites in mt-tRNAs from mESCs using CRACI. d Quantification of D stoichiometry in mt-tRNAs from mES cells. The identified D sites in Arabidopsis thaliana using CRACI: e ct-tRNAs, f mt-tRNAs, and g chloroplast tRNAs. h Detection of D sites in chloroplast rRNA at position U2467. Venn diagrams illustrating the overlap of D sites in ct-tRNAs across 3 species—human (HepG2), mouse (mESC), and plant (Arabidopsis thaliana): i D16/17, j D20, k D47, and l D20a sites. The D fraction of each site was calculated as the average of two biological replicates.
