Fig. 5: Preserved function and proliferation of adoptively transferred P25 CD4⁺ T cells upon gemcitabine (Gem) treatment.

A Naïve CD4⁺ T cells were isolated from the spleens of P25 mice (Thy1.1⁺) and adoptively transferred via i.v. injection to C57BL/6J naïve mice (Thy1.2⁺). At 1 day after transfer, mice were infected with Mtb (~350 CFUs). B CFUs in the lungs at 21 days post Mtb infection (n = 4). The graph shows the mean ± SD. Data were analyzed by two-tailed Mann–Whitney rank test. C, D The frequency and number of Thy1.2⁺ P25 cells or Thy1.1⁺CD4⁺ T cells among lung cells (n = 4). The graph shows the mean ± SD. Data were analyzed by two-tailed Mann–Whitney rank test. E, F The frequencies of Ki67⁺ and CD44⁺ cells among Thy1.2⁺CD4⁺ T cells or Thy1.1⁺ P25 cells were analyzed by flow cytometry and are summarized in the graph (n = 4). The graph shows the mean ± SD. Data were analyzed by two-tailed Mann–Whitney rank test. G Isolated lung lymphocytes were restimulated ex vivo with Ag85B peptides, stained, and assessed for Thy1.1⁺ P25 cells among lung cells (n = 4). The graph shows the mean ± SD. Two-tailed Mann–Whitney rank tests were used to compare groups. n.s. not significant, *p < 0.05. B–G The data are representative of at least two independent experiments. Source data are provided as a Source Data file.