Fig. 7: Potent efficacy of indisulam in NK cell competent mice.

a Tumors derived from C-MYC transgenic neuroblastoma model implanted into immunodeficient NSG mice, T and B cell-deficient Rag2^-/- mice and immune competent C57BL6 mice, with indicated therapeutic schedules. Graph generated by Biorender (www.biorender.com). b Individual tumor volume for each group treated with indisulam. NSG (n = 8), Rag2^-/- (n = 7), C57BL/6 (n = 6). c Kaplan-Meier survival curve for each group of mice treated with indisulam, with indicated p values for comparisons of each group. P value is calculated by Log-rank (Mantel-Cox) test. d Individual tumor volume for SK-N-AS xenografts implanted into Rag2^-/- mice treated with vehicle (n = 6). e Individual tumor volume for SK-N-AS xenografts implanted into Rag2^-/- mice treated with 25 mg/kg of indisulam (n = 6), for 5 days on and two days off for two weeks, indicted by the blue arrow. f Percentage of IFNγ-producing NK cells in c-MYC tumors treated with vehicle (n = 3) and indisulam (n = 3) for 3 days, analyzed by flow cytometry. Data are presented as Mean +/- SEM. P value is calculated by two-sided, unpaired Student t-test. g Schematic diagram shows the co-culture of NK92 and SK-N-AS cells in a ratio of 3:1 with or without a 24-hour pretreatment with 150 nM of indisulam. The live SK-N-AS cells are pre-stained by Incucyte Caspase-3/7 Green before mixed with NK92 cells. The release of the DNA dye and fluorescent staining of the nuclear DNA serves as the indicator of apoptosis. h Real-time quantification of apoptosis of SK-N-AS cells measured by the Incucyte Caspase-3/7 Green. The y-axis indicates the caspase-3/7 activity (apoptosis) while the x-axis indicates the elapsed time of co-culture of NK92 and SK-N-AS cells (n = 4 per group). Data are presented as Mean +/- SEM. P values calculated by two-sided, Student t-test for pairwise comparisons at the 12 h timepoint. ***p < 0.0001. i Kaplan-Meier survival for neuroblastoma patients with high and low expression of NCR1 gene in SEQC cohort (GSE62564)⁸³. P value calculated by Log-rank (Mantel-Cox) test. Source data are provided as a Source Data file.