Fig. 3: Ribonuclease 3-dependent bacterial-RNA species protect from polyQ40 protein aggregation.
From: Bacterial RNA promotes proteostasis through inter-tissue communication in C. elegans
a Quantification of polyQ40::YFP fluorescent foci of 2-day-old worms on OP50, OP50(xu363) and HT115 bacterial diets. The number of aggregates per worm is shown. Number of worms, n = 73 (OP50), n = 78 (OP50(xu363)), n = 82 (HT115). Values represent mean ± SD from three independent experiments. One-way ANOVA with Sidak’s multiple comparison test was used. OP50 vs. OP50(xu363) and OP50 vs. HT115 with P < 0.0001, OP50(xu363) vs. HT115 P = 0.9798, P < 0.0001. b Representative images of 2-day-old polyQ40::YFP-expressing worms on OP50, OP50(xu363) and HT115 bacterial diets. Scale bar is 100 μm. c Quantification of total polyQ40::YFP fluorescent foci of 2-day-old worms on OP50 and OP50(xu363) containing the empty vector (EV) or expressing the wt ribonuclease 3 (+rnC(wt)) and catalytically inactive ribonuclease 3 (+rnC(E117K), +rnC(E117D)). The number of aggregates per worm is shown. Number of worms, n = 54 (OP50), n = 67 (EV), n = 68 (+rnC(wt)), n = 65 (+rnC(E117K)), n = 61 (+rnC(E117D)). Values represent mean ± SD from three independent experiments. One-way ANOVA with Sidak’s multiple comparison test was used. All with P < 0.0001. d Representative images of 2-day-old polyQ40::YFP-expressing worms on OP50 and OP50(xu363) expressing the wt (+rnC(wt)) or catalytically dead (+rnc(E117K) or +rnC(E117D)) ribonuclease 3. EV serves as the control vector. Scale bar is 100 μm. e Quantification of polyQ40::YFP fluorescent foci of 2-day-old worms on wt and ribonuclease-depleted (rnC-) Nissle 1917 E. coli. The number of aggregates per worm is shown. Number of worms, n = 86 (WT), n = 82 (rnC-). Values represent mean ± SD from three independent experiments. Mann–Whitney t-test was used. P < 0.0001. f Representative images of 2-day-old polyQ40::YFP-expressing worms on wt and ribonuclease 3-depleted (rnC-) Nissle 1917 E. coli. Scale bar is 200 μm. g Representative images of 2-day-old poly-Q40::YFP-expressing worms on OP50 diets, descendants of worms in which their gonads were injected with water (H2O), DNA or HT115-derived RNA. Scale bar is 100 μm. Source data are provided as a Source Data file.
