Fig. 4: The RNAi machinery and the germline are required to protect from protein aggregation.
From: Bacterial RNA promotes proteostasis through inter-tissue communication in C. elegans
a Quantification of polyQ40::YFP fluorescent foci of 2-day-old worms on HT115, treated with empty vector (EV), sid-1(RNAi), sid-2(RNAi), rde-1(RNAi), ego-1(RNAi) and ergo-1(RNAi). The number of aggregates per worm is shown. Number of worms, n = 91 (EV), n = 51 (sid-1(RNAi)), n = 34 (sid-2(RNAi)), n = 54 (rde-1(RNAi)), n = 23 (ego-1(RNAi)), n = 41 (ergo-1(RNAi)). All with P < 0.0001. b–f Quantification of polyQ40::YFP fluorescent foci of 2-day-old worms on OP50, OP50(xu363) and HT115. The number of aggregates per worm is shown in sid-1(pk3321), number of worms, n = 43 (OP50), n = 38 (OP50(xu363)), n = 41 (HT115). OP50 vs. OP50(xu363) with P = 0.535, OP50 vs. HT115 P = 0.0724, OP50(xu363) vs. HT115 P = 0.9978 (b), sid-2(gk505), number of worms, n = 32 (OP50), n = 34 (OP50(xu363)), n = 42 (HT115), OP50 vs. OP50(xu363) with P = 0.8177, OP50 vs. HT115 P = 0.0346, OP50(xu363) vs. HT115 P = 0.0024 (c), rde-1(ne219), number of worms, n = 65 (OP50), n = 55 (OP50(xu363)), n = 47 (HT115) with OP50 vs. OP50(xu363) P = 0.2516, OP50 vs. HT115 P = 0.0263, OP50(xu363) vs. HT115 P = 0.0002 (d), rde-2(pk1657), number of worms, n = 43 (OP50), n = 41 (OP50(xu363)), n = 46 (HT115) with OP50 vs. OP50(xu363) P < 0.0001, OP50 vs. HT115 P < 0.0001, OP50(xu363) vs. HT115 P = 0.9642 (e) and ppw-1(tm5919), number of worms, n = 35 (OP50), n = 32 (OP50(xu363)), n = 31 (HT115) with OP50 vs. OP50(xu363) P < 0.0001, OP50 vs. HT115 P = 0.9965, OP50(xu363) vs. HT115 P < 0.0001 (f) strains. g Germline, number of worms, n = 45 (OP50), n = 44 (OP50(xu363)), n = 39 (HT115) with OP50 vs. OP50(xu363) P = 0.8742, OP50 vs. HT115 P = 0.0958, OP50(xu363) vs. HT115 P = 0.3607 and h intestine number of worms, n = 39 (OP50), n = 35 (OP50(xu363)), n = 41 (HT115) with OP50 vs. OP50(xu363) P = 0.0889, OP50 vs. HT115 P < 0.0001, OP50(xu363) vs. HT115 P = 0.0016, RNAi-specific mutant strains were used to quantify polyQ40::YFP fluorescent foci of 2-day-old worms of OP50, OP50(xu363) and HT115. The number of aggregates per worm is shown. Values represent mean ± SD from three independent experiments. One-way ANOVA with Sidak’s multiple comparison test was used. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.
