Fig. 5: Muscle function and neurotransmission protect against the accumulation of protein aggregates.
From: Bacterial RNA promotes proteostasis through inter-tissue communication in C. elegans
a–d Volcano plots of total quantified proteins showing significant increase or decrease in content in WT (a, c) and polyQ40-expressing (b, d) strains on OP50, OP50(xu363) or HT115 bacteria. In blue are the proteins with q values less than 0.05. UNC-89, UNC-22, TTN-1, UNC-15, ATN-1, UNC-54, UNC-87, ZK1321.4, Y43F8B.1, TNT-2, CPN-3, and CLIK-1 proteins from the STRING analysis are shown in red. Horizontal dotted lines are at −0.3, 0, and +0.3 fold change. e Venn diagram illustrating the intersections among proteins of worms grown on OP50, OP50(xu363) or HT115 bacteria. 194 proteins were significantly altered between OP50 and both HT115 or OP50(xu363)-fed worms. f STRING functional protein association network analysis of the upregulated proteins is shown. A cluster of 12 muscle-related proteins is formed. g–j Quantification of polyQ40::YFP fluorescent foci of 2-day-old worms on HT115, treated with empty vector (EV), unc-22(RNAi) (g), unc-89(RNAi) (h), unc-27(RNAi) (i) and unc-2(RNAi) (j). Number of worms, n = 118 (EV), n = 104 (unc-22(RNAi)) with P < 0.0001 (g), n = 63 (EV), n = 54 (unc-89(RNAi)) with P < 0.0001 (h), n = 49 (EV), n = 61 (unc-27(RNAi)) with P < 0.0001 (i), n = 45 (EV), n = 64 (unc-2(RNAi)) with P < 0.0001 (j). k–n Quantification of polyQ40::YFP fluorescent foci of 2-day-old worms on OP50, OP50(xu363) and HT115. The number of aggregates per worm is shown in unc-13(e1091) (k), unc-31(e928) (l), unc-38(e264) (m) and unc-29(e193) (n) mutant strains. Number of worms, n = 89 (OP50), n = 79 (OP50(xu363)), n = 89 (HT115) with OP50 vs. OP50(xu363) P = 0.0113, OP50 vs. HT115 P = 0.8484, OP50(xu363) vs. HT115 P = 0.804 (k), n = 100 (OP50), n = 108 (OP50(xu363)), n = 104 (HT115) with OP50 vs. OP50(xu363) P < 0.0001, OP50 vs. HT115 P < 0.0001, OP50(xu363) vs. HT115 P = 0.9996 (l), n = 42 (OP50), n = 42 (OP50(xu363)), n = 42 (HT115) with OP50 vs. OP50(xu363) P = 0.0106, OP50 vs. HT115 P = 0.0490, OP50(xu363) vs. HT115 P = 0.9298 (m), n = 43 (OP50), n = 41 (OP50(xu363)), n = 45 (HT115) with OP50 vs. OP50(xu363) P = 0.9996, OP50 vs. HT115 P = 0.0362, OP50(xu363) vs. HT115 P = 0.0501 (n). Values represent mean ± SD from three independent experiments. Mann–Whitney t-test (g–j) or one-way ANOVA with Sidak’s multiple comparison test (k–n) were used. ns P > 0.05, *P < 0.05, ****P < 0.0001. Source data are provided as a Source Data file.
