Fig. 7: Oligomeric state of MC4R in the presence or absence of MRAP2.
From: MRAP2 modifies the signaling and oligomerization state of the melanocortin-4 receptor
A Representative confocal micrographs of the basolateral membrane of HEK293 cells expressing SNAP-tagged GPCRs, all labeled using SNAP-surface647. From the top left the SNAP-β1AR (n = 4 transfections, 28 cells), SNAP-MC4R (n = 6 transfections, 135 cells), SNAP-MC4R in combination with eYFP-labeled MRAP2 (as a reference), SNAP-MC4R in presence of untagged MRAP2 (n = 4 transfections, 85 cells), SNAP-MC4Rchim (n = 3 transfections, 18 cells) and finally the SNAP-MC4R-H158R (n = 3 transfections, 35 cells). Scale bar is 10 µm. B Molecular brightness values obtained for each of the control groups listed in (A). Here, MRAP2 used to generate the micrographs of the SNAP-MC4R-WT + MRAP2 1:4 condition is untagged. Each dot represents the mean value from multiple regions of interest (ROIs) within a single cell. Several ROIs per cell were imaged. One-way ANOVA has been performed with Dunnett’s multiple comparisons post-hoc test (****: adjusted p-value < 0.0001). Mean ± SEM are shown in black.
