Fig. 3: DNA-PAINT with the smartphone-based microscope. | Nature Communications

Fig. 3: DNA-PAINT with the smartphone-based microscope.

From: Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope

Fig. 3: DNA-PAINT with the smartphone-based microscope.The alternative text for this image may have been generated using AI.

a 8HB DNA origami nanostructure with two docking sites and biotin surface binding functions. Super-resolved images of several nanostructures in an aqueous solution acquired with (b) the smartphone-based microscope and (c) a high-end microscope. Images are equally scaled. Scale bar: 2 µm. d One-to-one comparison of 10 nanostructures. Angle correspondence is observed. Scale bar: 300 nm. e Average 2D histograms of localizations of the same set of 10 nanostructures. Bin sizes are 25 nm for the smartphone-based microscope and 10 nm for the high-end microscope. Scale bar: 150 nm. f Localization histograms along the central axis (dashed white line) of the nanostructure in (e). Bin size: same as in (e). g Typical diffraction-limited fluorescence spots, i.e., the Point Spread Function of each system, equally scaled. Scale bar: 1 µm. The measurements presented in (b) and (c) were repeated six times with similar results. Source data are provided as a Source Data file.

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