Fig. 4: Super-resolution DNA-PAINT imaging on cells. | Nature Communications

Fig. 4: Super-resolution DNA-PAINT imaging on cells.

From: Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope

Fig. 4: Super-resolution DNA-PAINT imaging on cells.The alternative text for this image may have been generated using AI.

a Sketch of an immunolabeled microtubule with primary and secondary antibodies. The secondary antibody is conjugated with a docking DNA strand complementary to the Cy3B-labeled imager strand. b Super-resolution and diffraction-limited images of the microtubule network of fixed U2OS cells obtained with the smartphone-based microscope. The super-resolved image represents the localization density, while the diffraction-limited image is the actual fluorescence intensity. Scale bar: 2 µm. c, d Comparison of super-resolved images of the microtubule network obtained with the smartphone-based setup (c) – a magnified image of the region marked in (b) - and with the high-end microscope (d). Scale bar: 2 µm. e Line profiles across super-resolved microtubules as indicated in (c) and (d). Black solid lines: smartphone-based microscope. Pink dashed lines: high-end microscope. In total, four different videos were obtained in which the immunolabeled microtubules could be resolved with the smartphone-based microscope. Source data are provided as a Source Data file.

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