Fig. 1: Schematic representation of our Pan/Pf malaria workflow. | Nature Communications

Fig. 1: Schematic representation of our Pan/Pf malaria workflow.

From: Sensitive near point-of-care detection of asymptomatic and submicroscopic Plasmodium falciparum infections in African endemic countries

Fig. 1: Schematic representation of our Pan/Pf malaria workflow.The alt text for this image may have been generated using AI.

The integrated system combines the SmartLid whole blood extraction process with LAMP-based isothermal amplification and colourimetric readout. The SmartLid extraction process for malaria detection from finger prick whole blood involves a four-step workflow (Lysis, Wash 1, Wash 2 and Elution) including a 5-min heat-activated enzymatic incubation, and enables DNA purification and elution in under 10 min for a single sample. A total of 20 µL of eluted DNA is transferred using a fix-volume pipette into each reaction tube, followed by a maximum of 40-min incubation at 63.5 °C. Upon completion, LAMP results are qualitatively assessed by visually evaluating the colour change within the tubes, a pink colour indicating a negative result, while yellow a positive result. The validity of the test is confirmed by verifying that all controls exhibit the expected colour changes. Created in BioRender. Cavuto, M. (2025) https://BioRender.com/rwbh4tn.

Back to article page