Fig. 5: Inflammatory microglia expand during late C. neoformans infection. | Nature Communications

Fig. 5: Inflammatory microglia expand during late C. neoformans infection.

From: Brain-infiltrating CD4 T cells drive inflammatory microglia proliferation during cryptococcal meningitis in mice

Fig. 5

Analysis of single-cell RNA sequencing dataset from uninfected (n = 2) and infected (day 3, n = 2; day 6, n = 5) animals. a tSNE plot showing graph-based clustering results and 14 unique clusters identified within the data. b tSNE plots coloured by microglia, monocyte/macrophage or neutrophil signature genes. c tSNE plot coloured by time point analysed (day 0, grey; day 3, blue; day 6, red). d Proportion of sequenced cells from each of the analysed time points within microglia cluster 10 and all other identified microglia clusters in the dataset. e Heatmap of selected genes, grouped by function/similarity, showing mean expression by microglia cluster 10 or other microglia clusters. A full list of differentially-expressed genes is given in Data S1. f KEGG pathway analysis of upregulated genes by microglia cluster 10 (compared to other microglia clusters), showing gene ratio of detected genes within indicated pathways, P-value and enrichment scores. Statistics derived from Fisher’s exact test, with FDR multiple correction.

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