Fig. 1: Study design, de novo gene annotations and orthologous framework.
From: De novo annotation reveals transcriptomic complexity across the hexaploid wheat pan-genome

A Overview of transcriptome data generated for this study of wheat cultivars. 1 and 2: whole aerial organs sampled at dawn and dusk, 3: root, 4: complete spike at heading (GS59), 5: flag leaf 7 days post anthesis (GS71), 6: whole grains 15 days post anthesis (GS77). B De novo gene prediction results for each cultivar (left side, ‘genes’, separated for A, B and D subgenome) as well as summary of the BUSCO completeness assessment of gene models (right side, ‘BUSCO’). BUSCO genes found in two copies/duplicates are referred to as ‘exact_dupl’ and BUSCO genes found in more than three copies as ‘above_3’. C GENESPACE construction and visualisation of orthologous genes within the wheat cultivars, using de novo predicted gene models. Source data are provided as a Source Data file.