Fig. 1: Map-based cloning and functional validation of Pm68.
From: An NLR pair in the Pm68 locus confers powdery mildew resistance in durum and common wheat
a Genetic map of the region harboring Pm68 on the short arm of Durum wheat chromosome 2B. Pm68 was mapped to a 0.21 cM region flanked by markers Xdw05/Xdw06 and Xdw09/Xdw10. b Association mapping delimited Pm68 to 266-kb and 297-kb regions corresponding to Svevo and Zavitan reference genomes, respectively. Marker analysis of the 91 durum wheat accessions grouped them into nine different haplotypes, “n” indicates the number of accessions of each haplotype. Powdery mildew infection was evaluated using a 0–4 scale. 0; and 4 represent necrotic flecks and highly susceptible (no necrosis with full sporulation) reactions, respectively. Scale bar = 100 kb. c Comparation of Pm68 intervals from Svevo, Zavitan and TRI 1796 suggests the Pm68 interval of TRI 1716 is highly similar to Zavitan and diverged from Svevo. Synteny of genes from different accessions is indicated by dot lines. Scale bar = 20 kb. d Read-mapping depth of the Pm68 interval. RNA-seq data from BgtYZ01-infected leaf tissues of TRI 1796 were mapped to PacBio assembly of TRI 1796. Reads mapping data of the 305-kb Pm68 interval were extracted and then visualized by R package ggplot2. Expressed genes are indicated by blue and red colors. Two NLR genes are highlighted in red and their gene structures are represented as rectangular bars. Predicted protein domains are differentiated by color coding. CC, coiled-coil; NBS, nucleotide-binding site; LRR, leucine-rich repeat. Scale bar = 200 bp. e Responses of two independent F4 plants from crosses of different Pm68-1 and Pm68-2 T1 transgenic plants to Bgt isolate BgtYZ01 at the seedling and adult plant stages. The experiment was repeated three times with same results.
