Fig. 1: Map-based cloning of PmWR183. | Nature Communications

Fig. 1: Map-based cloning of PmWR183.

From: Paired NLRs originated from Triticum dicoccoides coordinately confer resistance to powdery mildew in wheat

Fig. 1: Map-based cloning of PmWR183.The alternative text for this image may have been generated using AI.

a Seedling and adult plant responses of parental lines WR183 and Fielder to Bgt isolate E20. Plant leaves were detached and photographed at 10 days post-inoculation (dpi) of the two-leaf (seedling) and 20 dpi of the jointing (adult) stage. Scale bar, 0.5 cm. R and S under pictures indicate the resistance and susceptibility of the plants to Bgt, respectively. Fungal structures of Bgt isolate E20 at 5 dpi (seedling stage) or 10 dpi (adult stage) were stained by Coomassie Brilliant Blue, respectively. Scale bar, 100 μm. b, c Genetic map of PmWR183 on the chromosome arm 2BS. d–f Physical map of PmWR183 locus on Fielder, contig ctg001568 of WE35, and Zavitan (WEW v1.0) reference genome, respectively. The red boxes indicate NLR genes. g Genomic structure and variations of PmWR183-NLR1 and PmWR183-NLR2 genes in WR183 and Zavitan. The nucleotide and corresponding amino acid change in PmWR183-NLR2 are shown as indicated. h The relative expression levels of PmWR183-NLR1 and PmWR183-NLR2 in WR183 at both seedling and adult plant stages under mock (uninoculated) and Bgt isolate E20 inoculation. Leaves from WR183 were collected 24 h after Bgt inoculation at both the two-leaf (seedling) and jointing (adult) stages. TaActin was used as the endogenous control. Data are means ± SEM (n = 3 biologically independent samples). Data were analyzed by two-tailed Student’s t-test (**p < 0.01, ***p < 0.001, ****p < 0.0001). Source data are provided as a Source data file.

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