Fig. 2: Structural basis of DNA binding by human MRN complex. | Nature Communications

Fig. 2: Structural basis of DNA binding by human MRN complex.

From: Structural basis for DNA break sensing by human MRE11-RAD50-NBS1 and its regulation by telomeric factor TRF2

Fig. 2: Structural basis of DNA binding by human MRN complex.

A, B Side by side comparison of the MRN-DNA-ADP•BeFx (sensing state) and MRN-ATPγS (resting state) complex structures. The α-helixes containing either the Walker A or signature motifs in both structures were colored in purple for better visualization. C Fluorescence anisotropy (FA)-based assay documenting the effect of ATP and ATPγS on MRN binding towards 64 bp Myb DNA. Data are presented as mean values+/− SD from three technical replicates. D–F Detailed molecular contacts between DNA and the MRN complex. For simplicity, only the nucleotides of the DNA template involved in the interactions were displayed as sticks. Black dashed lines represent electrostatic interactions or hydrogen bonds. Source data for C are provided as a Source Data file.

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