Fig. 1: DIGIT concept.
From: A Bayesian approach towards atomically-precise localization in fluorescence microscopy
a Farfield PSF of an emitter with a diffraction-limited width σ0 and its reconstructed location (μx, μy) using SMLM. b Probability density function f(μx) for a continuous variable x. c A zoomed-in view reveals the emitters' atomic structure, defined by a lattice with constant (a1,a2). Unlike a continuous spatial distribution, the emitters' positions are restricted to discrete sites determined by the underlying crystal lattice. d Comparison of posterior localization probability P(x) between conventional SMLM (purple) and DIGIT-equipped SMLM (green). e An exemplary DIGIT localization in two dimensions. The color coding in SMLM represents distinct lattice groupings. f Normalized localization precision σ as a function of total detected number of photons N: conventional SMLM follows the shot noise scaling (purple) while DIGIT exhibits exponential decay (green) when localization approaches lattice constant (σ ~ a).
