Fig. 3: Atomic-scale precision achieved by DIGIT.
From: A Bayesian approach towards atomically-precise localization in fluorescence microscopy
a Comparison of SMLM localization distribution f(x) in purple versus DIGIT posterior distribution P(x) in green, aligned along the lattice by gray dots. The inset illustrates the diamond lattice model projected along the [100] crystallographic direction, including lattice’s global rotation θ and offset U. b Normalized localization precision σ distance between all emitter pairs as a function of total collected number of photons N. The experimentally measured localization precision using DIGIT (green) with its simulation predictions (yellow), demonstrating an exponential improvement over the SMLM shot noise scaling (purple). The inset zooms in the transition where DIGIT localization begins to surpass conventional SMLM.
