Fig. 4: Large-scale DIGIT.
From: A Bayesian approach towards atomically-precise localization in fluorescence microscopy
a Three-dimensional representation of widefield PLE obtained by sweeping a resonant laser over 30 GHz in a 10 MHz increment. b Number of emitters in each reconstructed cluster. c Histogram of the measured precision σ for individually resolved emitters. d Top: Numerical simulations of the precision ratio σp/σf as a function of the number of measured photons N for different numbers of emitters M. Bottom: Representative experimental data with theoretical fits for M = 3, 4, 5. Dashed line: σp/σf = 1. e Top: Numerical simulations of the precision ratio σp/σf as a function of the number of measured photons N for various cluster sizes L, with M = 5 fixed. Bottom: Representative experimental data with theoretical fits showing the dependence on L ≥ 3000 and L ≤ 50. Dashed line: σp/σf = 1.
