Fig. 4: CryoEM structure of cannula-like tubules (Hyper2 thick tube) formed by the in vitro self-assembly of recombinant Hyper2. | Nature Communications

Fig. 4: CryoEM structure of cannula-like tubules (Hyper2 thick tube) formed by the in vitro self-assembly of recombinant Hyper2.

From: Donor strand complementation and calcium ion coordination drive the chaperone-free polymerization of archaeal cannulae

Fig. 4

a representative cryoEM micrograph and 2D-class average of recombinant Hyper2 tubes; b cryoEM map showing right-handed 8-start helical packing with C8 rotational symmetry (rise and twist of 11.16 Å and 9.59°, respectively); c helical model of Hyper2 thick tube; d local interaction network between neighboring Hyper2 subunits with three Ca2+ ions bridging protomers in a 12-mer segment of the cannulae; e close-up view of the three Ca2+ ions binding sites in stick representation. The representative image in this figure (a) is derived from a total of 5502 cryoEM micrographs from a single technical replicate.

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