Fig. 3: Intercellular transmission of Ca2+-induced ROS signaling from stressed plant leaves to roots.

a ROS changes in the root of plants leaf-sprayed with Thia and water (Control) using nitro-blue tetrazolium staining. Scale bar: 1 mm. b ROS contents in leaves and roots of plants under different organic pollutant exposures. c, d Contents of ROS and Ca2+ in plant tissues under different exposure times of Thia, respectively. e ROS contents in plant stems and roots after foliar application of Thia and lanthanum chloride (calcium antagonist). f Relative expression of BrRBOH genes in plant roots after foliar application of Thia for 6 hs. For (b–f), asterisks indicate statistically significant differences compared with the control or 0 h group (two-sided Student’s t-test, * for P < 0.05, ** for P < 0.01, *** for P < 0.001, **** for P < 0.0001; P values are shown in the Source Data file). Each data point represents the mean of replicates, and error bars represent standard deviations. For (b–e), each treatment includes 5 replicates. For (f), each treatment includes 3 replicates. Source data are provided as a Source Data file.