Fig. 4: miR-190a-5p is lost in pre-clinical models of kidney disease and is enriched in proximal tubular cells.

A miR-190a-5p expression was measured by RT-qPCR, normalised to U6 in RNA extracted from kidneys from mice culled 2 weeks after sham (n = 4, male mice) or uIRI surgery with contralateral nephrectomy (Nx, n = 6, male mice). Data are presented as mean normalised expression +/– SEM. Data analysed by Mann-Whitney test. **p = 0.0095 vs sham animals (B) miR-190a-5p expression was measured by RT-qPCR, normalised to U6 in RNA extracted from kidneys from mice culled 12 weeks post sham (n = 5, male) or subtotal nephrectomy (STNx) surgery to induce progressive kidney disease (n = 8, male). Data are presented as mean normalised expression +/− SEM. Data analysed by Mann Whitney test. * p = 0.0186 vs Sham animals. C Expression of miR-190a-5p determined by small RNA-seq performed on cell subsets isolated by FACS from the renal cortex of mice undergoing a reversible ureteric obstruction model. n = 16/cell type. Box (IQR) is the median, Q1 (25th percentile), Q3 (75th percentile). The whiskers represent: Minimum Q1-1.5*IQR and Maximum Q3-1.5*IQR. IQR = Interquartile range. D Small RNA-Seq of sorted cortical proximal tubular cells during the rUUO model. n = 3-4/gp. Box (IQR) is the median, Q1 (25th percentile), Q3 (75th percentile). The whiskers represent: Minimum Q1-1.5*IQR and Maximum Q3-1.5*IQR. IQR = Interquartile range (E). Correlation of miR-190a-5p expression and Tln2 in bulk cortical rUUO tissue. n = 20. Analysed by Pearson’s correlation. FPKM = Fragments Per Kilobase of transcript per Million mapped reads. CPM = Counts per Million.