Fig. 2: Characteristics of SFV-based VLP vector.
From: Engineering a streamlined virus-like particle for programmable tissue-specific gene delivery
a In vitro RNA and protein production duration after delivery. Protein synthesis and RNA copies were assessed at various time points post-delivery. The left y-axis illustrated protein synthesis in delivered cells through luminescence intensity, and the right y-axis indicates RNA copies in delivered cells. b In vitro delivery efficiency on human-derived cell lines. The luciferase mRNA containing vector’s delivery efficiency was evaluated at different vector genomic copies per cell ranging from 100 to 100,000. The y-axis depicted logged luminescence intensity in the delivered cells. c In vitro delivery efficiency on rodent-derived cell lines. The efficiency was assessed at varying vector genomic copies per cell from 100 to 100,000. The y-axis depicted logged luminescence intensity in the delivered cells. d Packaging capability for mRNA cargos. Different-length cargos (top) were packaged, and VLP genomic copies were quantified to represent packaging efficiency (bottom). (GFP vs. Cre, *P = 0.0163; Cre vs. spCas9-Csy4-sg *P = 0.0433). Statistical analyses were performed using one-way ANOVA with Bonferroni’s multiple comparison test. e VLP vector-mediated delivery of protein cargos in vitro. C protein or truncated C protein (Cd118) linked with luciferase by Furin (FUR) or Cathepsin L (CTSL) cleavable peptides. f Predicted structures of Luc-VLP. Luciferase was shown in bright orange. Furin cleavable peptides were shown in purple. C protein and truncated C protein were shown in sky-blue. The structures were predicted by AlphaFold2 ColabFold with MMseqs233,34. g Luminescence intensity of Luc-VLP at 10 ng per well was measured in delivered cells at different time points. Statistical analysis details were provided in Supplementary Data 1. h Negative-stain transmission electron microscopy (TEM) of VLP particles packaging mRNA with various sizes and protein cargos. Scale bar = 50 nm. All error bars represented the mean with standard deviation (SD) of three independent replicates. Source data are provided as a Source Data file.
