Fig. 4: Predicted metabolic capacity of Ca. ‘Aerarchaeales’.

Metabolic reconstruction of aerobic and anaerobic members within Ca. ‘Aerarchaeales’. Representative genomes CG-1 and YL-1 were used as reference for the metabolic cartoon to represent the Ca. ‘Aerovita’ and Ca. ‘Methanodefluvium’ lineage, respectively. Genes related to hydrocarbon and beta oxidation, the TCA/rTCA cycle, the oxidative phosphorylation chain, the Wood-Ljungdahl pathway, the methanogenesis pathway, and the electron transport chain are shown. Black or green arrows and text represent genes that were identified in both CG-1 and YL-1. Red or Blue arrows and text represent genes that were identified exclusively in CG-1 or YL-1, respectively. CuMMO particulate hydrocarbon monooxygenase, Sdh Succinate dehydrogenase Complex II, Fpo F420-dehydrogenase Complex I, bc1 Cytochrome bc1 complex, aa3 Cytochrome aa3 complex, Fix electron transfer flavoprotein fix complex, Etf electron transfer flavoprotein complex, Fd ferredoxin, F420 8-hydroxy-5-deazaflavin, NAD⁺ Nicotinamide adenine dinucleotide, NADP⁺ Nicotinamide adenine dinucleotide phosphate, NpdG NADPH-dependent F420 reductase, C c-type cytochrome, FpoF F₄₂₀H₂ dehydrogenase subunit F, FrhB coenzyme F₄₂₀-reducing [NiFe]-hydrogenase B subunit, HdrA heterodisulfide reductase subunit A, NiFe hyd [NiFe] hydrogenase. For full names and gene identifications in the CG-1 and YL-1 MAG, see Supplementary Data 4.