Fig. 6: αTIGIT-IL2 eliminates resistance to ICB treatment in TBNC, resulting in tumor regression. | Nature Communications

Fig. 6: αTIGIT-IL2 eliminates resistance to ICB treatment in TBNC, resulting in tumor regression.

From: αTIGIT-IL2 achieves tumor regression by promoting tumor-infiltrating regulatory T cell fragility in mouse models

Fig. 6

a Kaplan–Meier survival analysis of patients with TBNC based on the effector Treg score (mean expression of FOXP3, CTLA4, IFNG (inverted), TNF (inverted)). Data were acquired from The Cancer Genome Atlas (TCGA) and analyzed for significance by the log-rank test. b UMAP plot of individual datasets showing distinct Treg cell populations from responders or non-responders among patients with TBNC. c Gene Ontology enrichment analysis of scRNA-seq dataset in Treg cells for non-responders vs. responders post-ICB treatment. P values were computed with a one-sided hypergeometric test and adjusted for multiple comparisons using the Benjamini–Hochberg false discovery rate (FDR). d FOXP3 gene expression of Treg cells in the scRNA-seq dataset for non-responders vs. responders. e Treg functional score (mean expression of FOXP3, TIGIT, CTLA4, IL2RA, ENTPD1, ICOS, TNFRSF9, LAG3) expression level of Treg cells in the scRNA-seq dataset for non-responders vs. responders. f Treg functional score expression level of Treg cells in the scRNA-seq dataset from responder (left) and non-responder (right) for post-ICB treatment vs. pre-ICB treatment. g–i 6–8 weeks old female C57BL/6 J mice were inoculated with 2 × 105 EO771 cells. The tumor-bearing mice were administered the specified treatment intraperitoneally according to their respective groups (n = 5 individual animals/group) on days 7, 10, and 13. Tumor volumes (g, i) were measured as indicated, and growth curves for each individual animal (h) are shown. j 33 weeks old female C57BL/6 J mice from αTIGIT-IL2 + αPD1 treated tumor free mice (n = 2 individual animals) or 33w C57BL/6 J WT mice (n = 2 individual animals) were inoculated with 5 × 105 EO771 cells. Tumor volume was measured as indicated. k, l 6–8 weeks old female Balb/c mice were inoculated with 1 × 105 4T1 cells. The tumor-bearing mice were administered the specified treatment intraperitoneally according to their respective groups (n = 5 individual animals/group) on days 7, 10, and 13. Tumor volumes (k) were measured as indicated, and growth curves for each individual animal (l) are shown. Data represent mean ± SEM. The P value was determined by 2-tailed unpaired t-test (d–f) or 2-way ANOVA with Geisser-Greenhouse correction (g, i, k). Source data are provided as a Source Data file.

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