Fig. 4: Domain neighbourhoods, genomic organisation and metal binding analysis of Ang-1 and Ang-2 domains.
From: Signal-strapping as a protein-sequence search method for the discovery of metalloproteins
a Counts of function for the Ang-1 + Pfam13795 neighbour genes across bacterial genomes from the RefSeq database. b Counts of function for the Ang-2 + Pfam13795 neighbour genes across bacterial genomes from the RefSeq database. c Genomic organisation of Ang-1-containing operon in the genome of R. bacterium. d Genomic organisation of Ang-2-containing operon in the genome of M. maritypicum. e TSA of apo RbAng-1a in the presence of several metals at 1:1 molar equivalent. f TSA of apo MmAng-2a in the presence of several metals at 1:1 molar equivalent. Each TSA curve is coloured according with its respective metal-Cl2 as shown in the figure legend. Positive shifts indicate ligand binding to the proteins and thermal stabilisation, while negative shifts indicate ligand binding to the proteins and thermal destabilisation due to protein aggregation or precipitation. Source data are provided as a Source Data file.
