Fig. 2: Lipidomic analyses revealed that the different sterile mutant strains share similar but also distinct lipid profiles.

a Principal Component Analysis (PCA) analysis of three replicates of wild-type N2, glp-1(e2144), fem-3(e1996), and mog-3(q74) showed that the mutants are clearly separated from wild-type N2. Each dot indicates one replicate. PCA plot was generated using R. b Relative distribution of different lipids in N2, glp-1(e2144), fem-3(e1996), and mog-3(q74) worms showed that the major portions of the lipidome were comprised of TAG, PC, and PE and Sphingolipids, especially Ceramides. c, d Venn diagram showing upregulated lipid molecules (c) and downregulated lipid molecules (d) in glp-1(e2144), fem-3(e1996), and mog-3(q74) vs N2. Detailed lists of lipid molecules are provided in Supplementary Data 1. Fisher’s exact test was used to assess the significance of overlaps. **** indicates p-value < 0.0001. e Bubble plot showing lipid enrichment analysis. Circle diameter represents the enrichment score, while color intensity corresponds to the p-value. Statistical significance was assessed using one-sided Fisher’s exact test, without correction for multiple comparisons. See Supplementary Methods for details. PA Phosphatidic acid, CerG1 Glucosylceramide, TAG Triacylglyceride, SM Sphingomyelin, PC Phosphatidylcholine, PE Phosphatidylethanolamine, Cer Ceramide, LPC Lysophosphatidylcholine, MGDG Monogalactosyldiacylglycerol, PI Phosphatidylinositol, CL Cardiolipin, PS Phosphatidylserine, LPE Lysophosphatidylethanolamine, PG Phosphatidylglycerol, DG Diacylglycerol, So Sphingosine.