Fig. 4: Reduction of Sns to intermediate levels reveals shorter slit diaphragm (SD) lines, while the fishnet architecture of the SD remains.
From: The slit diaphragm in Drosophila exhibits a bilayered, fishnet architecture
a–c Fluorescence microscopy images acquired in Airyscan mode of nephrocytes after transient silencing of sns illustrate the reduction in the level of Sns (nephrin) protein, indicated by shortened lines of the SD. d–f Cryo-electron tomography confirmed the shorter SD lines, spanned by a fishnet pattern that is identical to the SD in wild-type nephrocytes. d 1 nm thick computational slice of a tomographic reconstruction illustrating a short SD line spanned by a fishnet-like SD. e Different region of interest of the same tomographic reconstruction as in (d), revealing the end or onset of two SD lines. f Zoom-in of the end/onset of the left line in (e). For Dot;Gal80ts>sns-RNAi nephrocytes, six tomograms displaying SDs could be acquired on 2 lamellae from two different Drosophila larvae on one electron microscopy grid.
