Fig. 2: Psilocybin does not treat social stress-induced impairments in maternal care.

A Timeline of experiment. Data illustrated in B–H represent maternal behaviors exhibited by control dams exposed to saline (n = 11) or psilocybin (n = 11), and stressed dams exposed to saline (n = 16) or psilocybin (n = 11) measured during a 1 h observation period 24 h following final intruder and psilocybin exposure on PPD 8 (shaded in A). Animals used in these experiments are the same as those used in Fig. 1. B Cumulative percentage of dams having retrieved their first pup to the nest following pup reunion. Percentage of observations made (C) in contact with the entire litter, (D) engaging in arched back nursing, (E) sniffing or licking their pups, (F) exhibiting stress-related behaviors, (G) avoiding litter in the cage not containing the nest. H Fragmented care score indicating behavioral switching between pup-directed and non-pup-directed behaviors. I Integrative maternal care behavioral risk score, an integrative measure of global maternal care impairments calculated by averaging z-normalized results from independent maternal care behaviors (arched back nursing, sniffing/licking, stress-related behaviors, avoidance and fragmented care), such that the greater the risk score, the greater the global impairment. Data in B are presented as a survival plot and comparisons between groups are made using a Log-rank Mantel–Cox test. Individual data in C–H are presented as aligned dot plots and as bar charts representing mean ± SEM. Given that there are a discrete number of observations able to be made in the 1 h observation period, the results from multiple dams may be represented by a single point on the graph. Data in I are presented as mean ± SEM. Statistical comparisons between experimental groups for data in C–I are made using a two-way analysis of variance. *p < 0.05, **p < 0.01. Exact P values are provided in the Source Data.