Fig. 1: Grpr is expressed in the dorsal and ventral striatum and Grpr+ cells in the NAc MSh have unique physiological properties.

a Representative image of a coronal brain section from a wild-type (WT) mouse (representative of 3 mice). Fluorescent in situ hybridization (FISH) for Grpr in magenta. Nuclei are labeled in blue with DAPI. Insets show higher magnification images for dorsal striatum (DS) and nucleus accumbens medial shell (NAc MSh). b Summary schematic of Grpr expression across the A/P axis of the DS and NAc MSh. Each circle represents the location of a Grpr+ cell. Cells are summed across 3 WT mice. c Representative image of a coronal brain section from a Grpr-eGFP mouse (representative of 6 mice). eGFP is in green, DAPI labeled nuclei are in blue. d Zoom-in of the NAc MSh from panel (c). e Zoom-in of panel d with eGFP in grayscale. f–i Representative traces from Grpr-eGFP+ (f, representative of 23 neurons), Grpr-eGFP- (g, representative of 21 neurons), D1-tdTomato+ (h, representative of 31 neurons), and D1-tdTomato-/Grpr-eGFP- (i, representative of 27 neurons) cells in the NAc MSh recorded at baseline (left) and following +40 pA current injection (right). j Mean ± SEM resting membrane potential of NAc MSh neurons. Dots represent values for individual cells. k Mean ± SEM membrane resistance of NAc MSh neurons. l Input-output curves showing the mean ± SEM firing frequency of NAc MSh neurons in response to positive current steps. m Mean ± SEM membrane potential for the first action potential (AP) evoked by current injection in each cell. Solid lines are the mean, shading represents SEM. For panels j and k, “+” indicates the presence of a fluorophore labeling the given cell type, “-“ indicates that the cell was negative for the fluorophore, and “o” indicates that the tissue did not have a fluorophore that would label the given cell type. Source data are provided as a Source Data file. See the Supplementary Data file for sample sizes and statistics.