Fig. 3: Subpopulations of Grpr+ neurons in the NAc MSh have distinct physiological properties.

a Representative image of the NAc MSh (representative of 8 mice). FISH for Casz1 in green and Grpr in magenta. Nuclei are labeled with DAPI in blue. Dashed circles outline Grpr+ cells. b Mean ± SEM percentage of Grpr+ cells in the NAc MSh and that are Casz1 + (n = 8 WT mice). c Mean ± SEM percentage of Casz1+ in the NAc MSh that are Grpr + (n = 8 WT mice). For b and c, dots represent values for individual mice. Values were obtained by counting all cells in the NAc MSh from two independent slices per mouse that were summed together. d Pair plot of passive properties from electrophysiology recordings from D1 (D1-tdTomato + ), D2 (D1-tdTomato-/Grpr-eGFP-), and Grpr (Grpr-eGFP + ) cells in the NAc MSh. Dots represent values for individual cells. Shaded regions represent the distribution of values for the indicated cell types. e UMAP of passive properties of D1, D2, and GRPR+ cells in the NAc MSh. Vm, Rm, and Cm values for each cell were centered and scaled to unit variance, and UMAP embeddings were computed using n_neighbors=10 and min_dist=0. Clusters were determined using the K-Means algorithm with n_clusters=4. f UMAP of passive properties from panel e colored by cell type. g Mean ± SEM resting membrane potential of NAc MSh neurons from the indicated groups. h Mean ± SEM membrane resistance. i Mean ± SEM membrane capacitance. j Mean ± SEM action potential (AP) height. k Mean ± SEM AP half-width. For g–k, dots represent values for individual cells. l Summary table of g–k. Data for D1+ and D2+ cells in g-i is the same as in Fig. 1, plotted here for comparison. Data for GRPR+ cells in g–i is the same as in Fig. 1 but subdivided by cluster. Source data are provided as a Source Data file. See the Supplementary Data file for sample sizes and statistics.