Fig. 4: CAMEL-GFP neurons identify the memory engram cells.
From: A memory transcriptome time course reveals essential long-term memory transcription factors
A Example of CAMEL-GFP (yellow) mushroom body co-stained with anti-Fasciclin II (magenta). Scale bar represents 50 μm. B Box plot showing CAMEL-GFP positive cells for naïve and trained flies of wt and Adcy1 mutant backgrounds. Statistical significance was determined using a two-tailed t-test, p = 0.015 for WT Naïve (WT-N; n = 11) vs WT Trained (WT-T; n = 16), and p = 0.7572 for Adcy1 Naïve (Adyc1-N; n = 11) vs Adcy1 Trained (Adcy1-T; n = 17). Box: 25th-75th percentile, whiskers: full data range, line: median Source data are provided as a Source Data file. C Box plot showing courtship indices (CIs) for CAMEL>eGFP (WT CAMEL) and CAMEL>TNTG for LTM assays 24 h after training. Statistical significance was determined using a two-tailed Mann-Whitney test p = 0.004 for WT CAMEL Naïve (WT-CAMEL N) vs WT CAMEL Trained (WT-CAMEL T), p = 0.214 for TNTG CAMEL naïve (TNT-CAMEL N) vs TNTG CAMEL Trained (TNT-CAMEL T). Number of flies tested for each condition is shown under corresponding dot plot. D Experimental scheme to isolate CAMEL positive MB neurons. Three experimental groups of flies were used: WT naïve (green), WT trained (blue) and Adcy1 mutant trained (red). WT and Adcy1 mutant trained animals were tested for 10 min, and 2 h later brains were dissected, dissociated, selected by FACs and sequenced (scRNAseq). E Box plot showing courtship indices (CIs) for the three experimental groups for LTM assays. Statistical significance was determined using a two-tailed Mann-Whitney test p = 0.0214 for WT Naïve vs WT Trained, p = 0.0459 for WT Trained vs Adcy1 Trained, and p = 0.3395 for WT Naïve vs Adcy1 Trained. Box: 25th-75th percentile, whiskers: full data range, line: median. Number of flies tested for each condition is shown under corresponding dot plot. Selected flies used for dissection and subsequent scRNAseq were shown as blue dots, with a Courtship Index below 0.72 or at least 3 unsuccessful courtship trials (threshold marked by a dotted line in the trained condition). Source data are provided as a Source Data file.
