Fig. 5: Analysis of scRNAseq data differentiates two memory related clusters.
From: A memory transcriptome time course reveals essential long-term memory transcription factors
A Principal Component Analysis (PCA) of WT trained flies with successful retrieval (K = 25). WTT-1 and WTT-2 are shown in light blue and purple, respectively. Each dot represents a neuron. B ey expression (white to red scale) in the PCA of WT trained flies. C PCA revealing the experimental condition for each cell: trained (WTT-1 and WTT-2), naïve (green) and trained Adcy1 (red). D The five clusters as defined by PCA (K = 15). E Composition of the five clusters by experimental conditions (naïve, Adcy1, WTT-1 and 2) by percentage. F Violin plots for general mushroom body and lobe marker genes for the five clusters. G Heatmap of the 10 most DEGs among the five clusters. The lower bar in the graph indicates the experimental condition for each sequenced neuron. H Most representative GO terms (FDR < 0.05) for the 4 and 5 clusters, indicating cell component (CC), biological processes (BP) and molecular function (MF).
