Fig. 1: Ultrafast photoacoustic microscopy of the multiScope.
From: A cortex-wide multimodal microscope for simultaneous Ca2+ and hemodynamic imaging in awake mice
a The schematic of the rotary scanning-based OR-PAM that suffers from inherent nonuniform sampling and photodamage in the central region. b Pixel-by-pixel ultrafast laser modulation used in the multiScope to achieve uniform sampling of OR-PAM in the imaging plane. c Spatial sampling density distribution map of the rotary scanning. The x-axis and y-axis represent the normalized position coordinates of the image, and the z-axis is the normalized oversampling factor. In addition, the oversampling factor-encoded MAP images are shown in the x-y plane. d Comparison of cerebral cortex imaging with conventional rotary scanning-based OR-PAM and the multiScope to simulate the thermal damage in the center of the FOV. The laser energy was set to 450 nJ to simulate the damage in the central position for long-term imaging. e The vessel mask derived from the pre-scan image after dilation processing with different sizes to avoid the influence of motion artifacts and vessel deformation during long-term imaging. The laser energy is 180 nJ (Supplementary Movie 1). f The pipeline of sparse sampling and upsampling reconstruction used in the multiScope.
