Fig. 2: The schematic and performance of the multiScope.
From: A cortex-wide multimodal microscope for simultaneous Ca2+ and hemodynamic imaging in awake mice
a In the mouse, the functional cortical areas are widely distributed. The multiScope with a FOV of about Ø8.6 mm can encompass the entire cortical neurovascular activity. b The schematic diagram of the multiScope. AL1–AL4, aspheric lens. DM1–DM4, dichroic mirror. F1–F4, filter. DL1–DL2, doublet lens. FC1–FC2, fiber collimator. CL concave lens, Galvo galvanometer scanner, SL scan lens, TL1–TL3 tube lens, CG cover glass, UST ultrasound transducer, P-SMF polarization-maintaining single-mode fiber, Obj1–Obj2 objective lens. c Illustration of the imaging interface for multimodal imaging of the cortex through a cranial window. The mice were head-fixed atop a circular treadmill (150 mm in diameter) with white and dark bars 10 mm apart. d The multiScope features a large FOV over 50 mm2. e The image of a 1951 USAF resolution test target captured by the multiScope. f The lateral resolutions of multiScope-OR-PAM across the FOV are estimated by the FWHMs of 1-μm-diameter Fe3O4 micro-beads. The resolution is 7.1 ± 0.8 μm (mean ± Std, n = 97). g The lateral resolutions of multiScope-Fluo across the FOV are estimated by the FWHMs of 1-μm-diameter fluorescence micro-beads. The resolution is 10.7 ± 3.1 μm (Mean ± Std, n = 121). h–j Representative normalized images of the cortex from widefield Ca2+ fluorescence microscopy, OR-PAM, and LSCI, respectively. Scale bar: 1 mm. Source data of Fig. 2f, g is provided as a Source Data file.
