Fig. 3: Simultaneous multimodal imaging during anesthesia and recovery.
From: A cortex-wide multimodal microscope for simultaneous Ca2+ and hemodynamic imaging in awake mice
a Averaged fluorescence signal (after hemodynamic calibration). b Averaged HbT signal during isoflurane-induced anesthesia. c Averaged \({\mbox{CBF}}v\) signal. The blue bars indicate the time for anesthesia. The solid lines in (a–c) are the mean data, and the shaded curves represent the range of signal fluctuations (from the minimum to the maximum). d–f Multimodal images show the changes in neural activity (\(\Delta {{{\rm{Fluo}}}}/{{{\rm{Fluo}}}}\)), total hemoglobin (\(\Delta {{{\rm{HbT}}}}/{{{\rm{HbT}}}}\)), and cerebral blood flow velocity (\({{{\rm{CBF}}}}v\)) distributed in the cortex. The fluorescence images before and after image-to-image hemodynamic calibration are shown in Supplementary Fig. 11. Scale bar: 1 mm. g Atlas regions of interest (ROIs) within the cortex used in this study. A total of 26 ROIs are distributed across both hemispheres. h Two bands (Low: 0.005–0.02 Hz; High: 0.04–0.1 Hz) of multimodal temporal traces of neural activity (\(\Delta {{{\rm{Fluo}}}}/{{{\rm{Fluo}}}}\)), total hemoglobin (\(\Delta {{{\rm{HbT}}}}/{{{\rm{HbT}}}}\)), and cerebral blood flow velocity (\(\Delta {{{\rm{CBF}}}}v/{{{\rm{CBF}}}}v\)). i–k Connectivity matrices in awake (i), anesthetized (j), and recovery (k) process across modalities of low (upper triangular part) and high (lower triangular part) components in multimodal time traces derived from 26 ROIs. Source data of Fig. 3a–c, h are provided as a Source Data file.
