Fig. 6: Inactivation of Taf4a abrogates skeletal muscle regeneration.
From: Regulation of NSL by TAF4A is critical for genome stability and quiescence of muscle stem cells
a Immunofluorescence staining of EdU/PAX7 double-positive MuSCs on FDB myofibers from control and Taf4asKO mice, cultured for 3 days. Scale bar: 20 µm. Quantification of relative numbers of EdU + /PAX7+ cells found on 50 myofibers (unpaired two-tailed t test: * p = 0.0377, n = 4). b Quantification of Pax7+ colonies per 50 myofibers (unpaired two-tailed t test: *p = 0.0244, n = 4). c Quantification of EdU-incorporation in cultured control and Taf4asKO MuSCs (unpaired two-tailed t test: **p = 0.0044, n = 3). d Immunofluorescence staining of PAX7-positive MuSCs in control (n = 5) and Taf4asKO (n = 6) TA muscle sections. Scale bar: 20 µm. Quantification of PAX7+ cells per mm2 section area is in the right panel (unpaired two-tailed t test: ***p = 0.0002). e TUNEL assay of cultured control and Taf4asKO MuSCs. Scale bar: 10 µm. Quantification of TUNEL-positive cells is shown on the right (unpaired two-tailed t test: **p = 0.0034, n = 4). f Western blot analysis of p53 expression in cultured MuSCs from control and Taf4asKO mice. H3 was used as loading control (n = 2). g Outline of the muscle regeneration assay. Representative macroscopic views of TA muscles from control and Taf4asKO mice 14 days after muscle injury. Scale bar: 1 mm. Ratio of TA muscle masses with and without injury in control and Taf4asKO mice (unpaired two-tailed t test: **p = 0.0017, n = 3). h, i H&E (h) and Trichrome staining (i) of TA muscle sections from control and Taf4asKO mice 14 days after injury (n = 3). Scale bar: 20 μm. Myofibers and MuSCs were isolated 4–6 weeks after tamoxifen administration in (a–f). Muscles were isolated 2 weeks after tamoxifen administration in (g–i). 8–20-week-old males and females were used. Data are presented as mean ± SEM of biological replicates. Source data are provided in the Source Data file.
