Fig. 2: The basic SMALL YES gate. | Nature Communications

Fig. 2: The basic SMALL YES gate.

From: DNA computing function switching by programming base stacking interactions with minimal molecular architecture changes

Fig. 2: The basic SMALL YES gate.The alternative text for this image may have been generated using AI.

a Illustrations of the basic SMALL YES gate module. b The design details of the basic SMALL YES gate regulation module. c Schematic illustration of the stacking interactions adjusting in the module. d Histogram of the fluorescence results of the SMALL regulations with different stacking interactions (140 min, mean ± SD, n = 3 from independent experiments, *p ≦ 0.05, **p ≦ 0.01, ***p ≦ 0.001). e Fluorescence results of the basic SMALL YES gate with the regulation of stacking interaction 1. complex (A/B1/C) = 0.2 μM and D1 = 0.2 μM. a.u.: arbitrary units (mean ± SD, n = 3 from independent experiments). f Native PAGE analysis of the basic SMALL YES module. Lane 1, A; lane 2, C; lane 3, B2; lane 4, complex (A/C); lane 5, DNAzyme-1 (B2/C); lane 6, complex (A/B2/C); lane 7, complex (A/B2/C) + D2. All DNA strands were used at 800 nM in reactions conducted at 25 °C. The PAGE experiment was independently repeated three times, yielding similar results. g Fluorescence results using different input D2 concentrations of 0×, 0.2×, 0.4×, 0.6×, 0.8× and 1.0×, with 1× as 200 nM (mean ± SD, n = 3 from independent experiments). Source data are provided as a Source Data file.

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