Fig. 3: Dissolution of chiral coacervate via intrinsic catalysis.

Time-dependent a brightfield microscopy and corresponding b CLSM images with fluorescein dye (10 μM) of 1 + A coacervate droplets. c Size distribution plot of 1 + A coacervate droplets (violin plot was prepared from the CLSM images at different time using ImageJ software, the number at each violin and green square represents number of droplets and median respectively, increment in droplet size observed after 60 min could be attributed to the formation of vacuoles and coalescence). d 3D reconstruction of the vacuole formed at 120 min. e CLSM image and f intensity profile across the vacuole formed at 120 min (dotted white line, scale bar 5 μm). g Brightfield and corresponding h CLSM images showing fusion of two vacuoles with time within 1 + A coacervate droplet. i Representative time-dependent HPLC chromatogram of 1 + A coacervate system. j Plot to show the change in A and A’ concentration with time. (Error bars represent the standard deviation of triplicate experiments). [1] = 40 mM, [A] = 10 mM, HEPES buffer (pH 8, 80 mM).