Fig. 1: Functional characterization and structure of human G6PT.
From: Structural basis for transport and inhibition of the human glucose-6-phosphate transporter G6PT
a [13C] glucose-6-phosphate (G6P) uptake by HEK293F cells expressing human G6PT, co-expressing G6PT and G6PT-ALFA with G6PC1 and inhibition of [13C]G6P uptake by CHA. Cells expressing GFP and co-expressing GFP with G6PC1 served as controls. Data were presented as mean values ± SEM; n = 6 independent replicates for GFP + G6PC1 and G6PT + G6PC1, n = 3 independent replicates for all other conditions. One-way ANOVA was used. nsP = 0.9844 for G6PT + G6PC1 vs. G6PT-ALFA + G6PC1, **P = 0.0091 for GFP vs. G6PT, ****P < 0.0001. b The size-exclusion chromatography profile of purified G6PT-ALFA in complex with NbALFA. The peak fraction was visualized by SDS-PAGE with Coomassie blue staining. The gels presented here were made from an original gel without changing the relative size and height of them. The fSEC profile and gel image are representative of 3 experimental replicates. c The EM map of G6PT-ALFA embedded in LMNG micelle (gray), viewed parallel to the membrane plane (left) and perpendicular to the membrane from the cytosol (right). The N- and C-domains of G6PT are colored in yellow and blue, respectively. d, e The cartoon representation of human G6PT structure, viewed parallel to the membrane plane (d) and perpendicular to the membrane from the cytosol (e). Distance is indicated by a red bidirectional arrow. The angle between the N- and C-domains of G6PT is shown with dashed lines. Source Data are provided as a Source Data file.
