Fig. 2: Structural basis of G6P recognition by G6PT.
From: Structural basis for transport and inhibition of the human glucose-6-phosphate transporter G6PT
a The chemical structure of G6P. b The EM map of G6PT-G6P embedded in LMNG micelle (gray). The N- and C-domains of G6PT are colored in yellow and blue, respectively. c The cartoon representation of G6PT. G6P is shown in cyan sticks. Distances are indicated by red bidirectional arrows. d The electron microscopy densities of G6P and nearby residues. The EM densities for G6P and the interacting residues were contoured at 4.5 σ. The density of G6P and nearby residues is shown in red and blue meshes, respectively. e Cut-open electrostatic potential surface of G6PT-G6P, viewed parallel to the membrane plane (left) and perpendicular to the membrane from the ER lumen (right). The electrostatic potential surface was calculated in PyMoL (red to blue, −50 kT/e− to +50 kT/e−). The red arrow indicates G6P. W118, W138, and H366 are shown in pink sticks. f, g Detailed interactions between G6P and G6PT. Residues involved in G6P-binding are shown as sticks. Potential polar interactions are indicated in purple dashed lines. h [13C]G6P uptake by HEK293F cells co-expressing G6PT and G6PT mutants with G6PC1. Cells co-expressing GFP with G6PC1 served as controls. Data were presented as mean values ± SEM; n = 12 independent replicates for G6PT-WT, n = 3 independent replicates for all other mutants. One-way ANOVA was used. ****P < 0.0001. Source Data are provided as a Source Data file.
