Fig. 6: EF-heMSCs form Ewing-type sarcomas in vivo. | Nature Communications

Fig. 6: EF-heMSCs form Ewing-type sarcomas in vivo.

From: EWS::FLI1 expression in human embryonic mesenchymal stem cells leads to transcriptional reprograming, defective DNA damage repair and Ewing sarcoma

Fig. 6

A H&E staining of representative lesions from NOD/SCID mice injected in the gastrocnemius with EF-heMSCs. Images show representative sections at two different magnifications (top bars, 100 µm; bottom bars, 20 µm). Observe the aberrant mitotic figure and the presence of blood lakes shown in the insets of the abdominal tumor masses. B IHC to detect human HLA. Magnification bars: 50 μm. C IHC to detect human HLA and human Ku80 in sequential sections of the same sample. Magnification bars: 50 μm. D IHC of human HLA and Flag in sequential sections of EF-heMSC samples. Magnification bar: 50 μm. E IHC of PRKCB, BRCA1, and hHLA in serial sections of EF-heMSC-derived tumors. Lung and thymus sections correspond to normal tissues. Magnification bars: 50 μm. N = 7 tumors.

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