Fig. 5: Validation of R-eLACCO2.1 in awake mice.
a Schematic illustration of in vivo mouse imaging upon i.v. l-lactate injection. b Fluorescence response traces (left) of the primary vibrissa somatosensory (vS1) cortex in an awake mouse expressing hSyn-R-eLACCO2.1 upon i.v. injection of l-lactate (4 mmol kg-1). Mean ± s.d. (n = 30 neurons). The right panels show representative images of F and ΔF/F at the time marked in yellow. Scale bars, 100 μm. c Schematic illustration of in vivo imaging for a calibrated EC l-lactate probe in awake mice. d Signal traces of EC l-lactate probe (left), and R-eLACCO2.1 and R-deLACCOctrl (right) using AO2P. hSyn-R-eLACCO2.1 and hSyn-R-deLACCOctrl were simultaneously recorded in a field of view (right bottom) of an awake mouse. Scale bar, 100 μm. e Correlation plot between the signals of R-eLACCO2.1 (4 mice) and EC probe (3 mice) in awake mice upon i.v. injection of l-lactate. Mean ± s.d. The linear fit is overlaid with a slope of 0.41, an intercept of 0.03, and an R2 of 0.9. f Fluorescence response traces of the layer I cortex in awake mice expressing hSyn-R-eLACCO2.1 (excited at 1,100 nm) or hSyn-eLACCO2.1 (excited at 930 nm) upon i.v. injection of l-lactate (4 mmol kg-1) or saline using AO2P (3 mice for each biosensor). Two-sided ANOVA with Dunnett’s post hoc tests. *p = 0.0206. Source data are provided as a Source Data file.
