Fig. 3: Phylogenetic analysis of ITS2 amplicons derived from Trichuris-positive stool samples of patients from Côte d’Ivoire, Laos, Tanzania, and Uganda.

Maximum likelihood tree based on the ITS2 rDNA using Tamura-Nei with gamma distribution as the substitution model and Trichinella spiralis (GenBank accession: KC006432) as an outgroup. Colored branches correspond to major clades A to E, which broadly align with WGS-derived sequences from T. trichiura, T. incognita, T. suis, T. muris, and livestock-derived reference sequences. The external rings (from inner to outer) are colored according to metadata, including host type, country of origin, and whether the sequences were generated in this study or obtained from a public database. The host silhouettes (human, livestock, rodent, non-human primates) supporting the phylogenetic tree were created in BioRender. Pierre, S. (2025) https://BioRender.com/6cv1f89.