Fig. 6: Fragment length could distinguish between different human-infecting Trichuris species.

a Comparison of the fragment length between the five clades; Clade A (n = 9), Clade B (n = 31), Clade C (n = 17), Clade D (n = 532), and Clade E (n = 653) was conducted using pairwise two-sided Mann–Whitney tests with Benjamini–Hochberg adjustment for multiple comparisons. Boxplots show the median (centre line), interquartile range (box bounds = 25th and 75th percentiles), and whiskers indicating the 95% CI. b ROC curve showing the performance of a random forest model built using ITS2 fragment length, evaluated with a leave-one-out cross-validation (LOOCV) approach. c Confusion matrix showing the class distribution of ITS2 fragment lengths from each Trichuris species. bp: base pair.