Table 2 Genetic diversity estimations of Trichuris population per region

Country	Species	ASV clusters (N)	Nucleotide diversity (π)	Tajima’s D	Fu and Li’s D	Fu and Li’s F
Côte d’Ivoire	T. trichiura	84	0.021	−2.784**	−6.633**	−5.994**
Côte d’Ivoire	T. incognita	40	0.017	−2.567***	−4.278**	−4.252**
Laos	T. trichiura	57	0.012	−2.525**	−4.609**	−4.496**
Laos	T. incognita	12	0.027	−1.897*	−2.111*	−2.083*
Tanzania	T. trichiura	58	0.011	−2.541	−4.939	−4.748
Tanzania	T. incognita	11	0.012	−2.111**	−2.570**	−2.463**
Uganda	T. trichiura	55	0.015	−2.725***	−5.240**	−5.025**
Uganda	T. incognita	14	0.020	−1.952*	−2.322	−2.314*

Genetic diversity metrics, including Tajima’s D and Fu and Li’s F* and D* statistics, were calculated using DNASP software. Significance testing in DNASP is based on coalescent simulations under the neutral model. The reported p-values are approximate and represent two-sided tests. ASV Amplicon Sequence Variant; *p < 0.05; **p < 0.01; ***p < 0.001 (statistical significance levels).

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