Fig. 2: Extraction, sequence and localization of mussel stem protein-1 (MSP-1). | Nature Communications

Fig. 2: Extraction, sequence and localization of mussel stem protein-1 (MSP-1).

From: A dynamic biointerface in mussels mediated by a mechanoresponsive intermediate filament-based biopolymer

Fig. 2: Extraction, sequence and localization of mussel stem protein-1 (MSP-1).The alternative text for this image may have been generated using AI.

a Silver staining of an SDS-polyacrylamide gel displays the band representing the protein extracted from the stem root lamellae (N = 3 gels from 3 specimens). b Western blot of the extracted protein stained in (N = 2 Western blots from 2 specimens) (a) using an antibody raised against MSP-1 peptide (N = > 30 images from 5 specimens). c Trichrome-stained section of the generator in which an unstretched stem root is embedded (blue: collagenous, red: non-collagenous components) (N = > 30 images from 5 specimens). d Immunostaining fluorescence image collected with SIM of a single stem root lamellar sheet and surrounding tissue; blue: nuclei, green: cilia, red: MSP-1 (N = > 30 images from 5 specimens). e A schematic of the protein sequence with annotations describing the characteristic features of MSP-1. f AlphaFold-predicted conformational structure of MSP-1. Source data are provided as a Source Data file.

Back to article page