Fig. 1: Generation of early postnatal developmental mouse brain atlas (epDevAtlas).

a Symmetrical, morphology and intensity averaged templates of the early postnatally developing mouse brain at postnatal (P) days 4, 6, 8, 10, 12, and 14 using samples from STPT imaging. Sample sizes per template: P4 (n = 6), P6 (n = 8), P8 (n = 4), P10 (n = 7), P12 (n = 6), P14 (n = 5). b Anatomical labels from the adult Allen CCFv3 were registered to each developmental time point in a stepwise manner. c Cortical layer-specific cell subclass labeling using genetic strategies was implemented to refine and validate anatomical labels (L1 = layer 1, etc.). Cells are labeled by these transgenic lines by the abbreviated name of the driver lines (Table 1). Representative STPT coronal brain images shown are all from P10 animals. d–g Examples of anatomical brain region delineations via cell type-specific labeling from (d) Gad2, (e) Vip, (f) Sst, and (g) Pvalb mice, collectively guiding epDevAtlas annotations. h Volumetric brain growth chart during early postnatal mouse development. All brain region volumes (mm³) are reported as the mean ± standard deviation (s.d.; shaded area between error bars) (total n = 38). Source data are provided as a Source Data file. Additional abbreviations: CB, cerebellum; CP, caudoputamen; GPe, external globus pallidus; IC, inferior colliculus; PAG, periaqueductal gray; SCH, suprachiasmatic nucleus.